Adult dorsal root Eaux De Toilette ganglia neurons are among the few adult neuronal cell types that can be purified and grown relatively easily in dissociated cell culture.Here we describe a procedure for the isolation and growth of dissociated adult mouse DRG neurons using Percoll RELAXANT gradients and a chemically defined medium.These cultures can be used for morphological, biochemical and electrophysiological studies of neuronal growth and function.